Clay-reinforced PVC composites and nanocomposites.

Clay-reinforced polyvinyl chloride (PVC) composites and nanocomposites are one of the newest and most important compounds studied and used in various applications, including the biomedical, automotive industry, water treatment, packaging, fire retarding, and construction. The most important clays used in the synthesis of these composites are Bentonite, Montmorillonite, Kaolinite, and Illite. The addition of these nanoclays to the PVC matrix improves mechanical properties, thermal stability, and yellowness index properties. In this chapter, a detailed study of PVC and its properties, types of nanoclays and their properties, modification of nanoclays, production methods of composites, and nanocomposites of PVC/clay, their characterization, and applications have been performed. Herein, the types, properties, and applications of PVC/clay nanocomposites, as well as their challenges and future remarks, are reviewed.

Applying the theory of planned behavior for prediction of oocyte donation intention among Iranian women.

BACKGROUND: Oocyte donation is a complex and multifaceted behavior in women. Due to the acute deficiency of donated oocytes, it is necessary to identify the factors affecting the desire to donate. Planned behavior theory is a suitable model for explaining and predicting behavior in many behavioral domains. The aim of this study was to predict oocyte donation intention using the theory of planned behavior. METHODS: This cross-sectional study was performed on 556 women who were covered by the comprehensive health service centers in Gonabad and Neyshabour cities in 2020. Sampling was performed by the two-step method in Gonabad city and by the convenience sampling method in Neyshabour city. Data were collected online using researcher-made questionnaires included oocyte donation awareness questionnaire and oocyte donation intention questionnaire (based on the TPB constructs). The validity of the questionnaires was confirmed by face and content validity and its reliability was confirmed by Cronbach's alpha coefficient. Data were analyzed in SPSS software (version 16) using hierarchical linear regression. The statistical significance was considered as P < 0.05. RESULTS: The results showed that there was a significant positive correlation between the oocyte donation intention with all constructs of planned behavioral theory (P < 0.050). Planned behavior theory constructs explained 47% of the variance of oocyte donation intention. In addition, constructs of perceived power (ß = 0.461), control beliefs (ß = 0.154) and normative beliefs (ß = 0.125) were the strongest predictors of oocyte donation intention, respectively. CONCLUSION: Planning and implementing educational courses according to the constructs of the theory of planned behavior can be a great step toward the elimination of oocyte deficiency and infertility problems. Also performing of intervention or training strategies in the field of oocyte donation requires the enhancement of social norms and perceived behavioral control in the population under study.

Translation, cross-cultural adaptation, and validation of the Persian version of the Attitude Towards Menopause scale.

OBJECTIVES: This study describes translation, cross-cultural adaptation, and validation of the Attitude Towards Menopause (ATM) scale in Persian. METHODS: A standard process was followed for translation and cross-cultural adaptation of the ATM scale into Persian. The content validity index and content validity ratio were used to measure content validity. The construct validity was tested using exploratory factor analysis and confirmatory factor analysis. The average variance extracted and construct reliability were used to indicate convergent and discriminant validity, respectively. Internal consistency and test-retest reliability of the Persian ATM scale were assessed using Cronbach α and intraclass correlation coefficient (ICC). RESULTS: A revised version of the ATM scale, including 26 items assigned to seven factors, was obtained in exploratory factor analysis. The factors were interpreted as negative affect, postmenopausal recovery, control of symptoms, sexuality, psychological losses, unpleasant confrontation, and menstrual freedom, explaining 66.18% of the total variance. The factor structure of the scale was confirmed using confirmatory factor analysis (Comparative Fit Index = 0.90, Tucker-Lewis Index = 0.88, Goodness-of-Fit Index = 0.90, Adjusted Goodness-of-Fit Index = 0.86, Root Mean Square Error of Approximation = 0.07, χ2 / df = 4.56). The values of Cronbach α coefficient and ICC indicated an acceptable level of reliability ( α = 0.70, ICC = 0.89). The construct reliability for all factors was more than or equal to 0.7 except for psychological losses, indicating good discriminating validity. The values of average variances extracted for subscales varied from 0.48 to 0.99, representing adequate convergent validity. CONCLUSIONS: The Persian version of the ATM scale is a reliable and valid tool to evaluate the attitudes of Iranian women toward menopause.

Adsorption kinetics of methylene blue from wastewater using pH-sensitive starch-based hydrogels.

In this work, starch/poly(acylic acid) hydrogels were synthesized through a free radical polymerization technique. The molar ratios of acrylic acid to N,N'-methylenebisacrylamide were 95:5, 94:6, and 93:7. The samples exhibited an amorphous porous structure, indicating that the size of the pores was contingent upon the amount of cross-linking agent. The quantity of acrylic acid in structure rose with a little increase in the amount of the cross-linking agent, which improved the hydrogels' heat stability. The swelling characteristics of the hydrogels were influenced by both the pH level and the amount of cross-linking agent. The hydrogel with a ratio of 94:6 exhibited the highest degree of swelling (201.90%) at a pH of 7.4. The dominance of the Fickian effect in regulating water absorption in the synthesized hydrogels was demonstrated, and the kinetics of swelling exhibited agreement with Schott's pseudo-second order model. The absorption of methylene blue by the hydrogels that were developed was found to be influenced by various factors, including the concentration of the dye, the quantity of the cross-linking agent, the pH level, and the duration of exposure. The hydrogel 95:5 exhibited the highest adsorption effectiveness (66.7%) for the dye solution with a concentration of 20 mg/L at pH 10.0. The examination of the kinetics and isotherms of adsorption has provided evidence that the process of physisorption takes place on heterogeneous adsorbent surfaces and can be explained by an exothermic nature.

The relationship between women's sexual function and type of delivery and pelvic organ prolapse: A cross-sectional study.

ABSTRACTsThe probable relationship between type of delivery and postpartum sexual function is one of the reasons behind women's tendency to cesarean. This study was performed with 250 women participants. Female Sexual Function Index and examination form were used to determine pelvic organ prolapse and its severity and type based on POP-Q. Our results showed the difference between the mean performance score in the dimensions of desire (p = .19), lubrication (p = .08), orgasm (0.13), pain (p = .08), and satisfaction (p = .06) was not significant in the two groups of vaginal delivery and cesarean, but the difference between the mean score of sexual function in the dimension of total score (p = .002) was significant in the two groups. Regarding the relationship between sexual function and pelvic organ prolapse, the total score of sexual function in women with uterine, cystocele, and rectocele prolapse was significantly lower (p < .001). Researchers should work to provide more evidence on relationship of female sexual function and the type of delivery and pelvic organ prolapse.

Association between bacterial vaginosis and 25-Hydroxy vitamin D: a case-control study.

INTRODUCTION: Bacterial vaginosis (BV) is the most common vaginal dysbiosis among women of reproductive age. Micronutrient deficiencies, including vitamin D deficiency, can increase the risk of BV. The findings of previous studies regarding the relationship between vitamin D deficiency and BV were conflicting. Therefore, this study aimed to evaluate the association between BV and serum level of 25-hydroxy vitamin D. MATERIALS AND METHODS: This case-control study was conducted in Gonabad County in 2021. One hundred and twenty-five confirmed BV cases and 125 controls who were matched based on age and intercourse frequency (maximum difference of two days per week) enrolled in the study. Data collection was performed using a demographic and reproductive data questionnaire and a checklist for recording Whiff test results, serum 25-hydroxy vitamin D level, litmus paper observation, and microscopic findings (clue cells). Serum level of vitamin D was evaluated based on enzyme-linked immunoassay method (Monobind kit) from 0.5 ml venous blood drawn from each participant. The conditional logistic regression model was used to analyze data. RESULTS: The BV cases had significantly lower 25-hydroxy vitamin D serum levels than controls. The odds of BV increased with vitamin D deficiency (Adjusted odds ratio (AOR): 4.34, 95% confidence interval (CI): 1.39-13.4, p = 0.011, FDR q-value = 0.051), vitamin D insufficiency (AOR: 3.65, 95% CI: 1.23-10.85; p = 0.020; FDR q-value = 0.053), cigarette/hookah smoking (AOR: 3.65, 95% CI: 1.23, 10.85; p = 0.020; FDR q-value = 0.053) and lower age at first intercourse (AOR: 1.16, 95% CI: 1.05, 1.28; p = 0.004; FDR q-value = 0.048). The odds of BV was 0.80 and 0.78 times lower in participants who had coitus interruptus (AOR: 0.20, 95% CI: 0.06, 0.63; p = 0.006; FDR q-value = 0.048) and condom use (AOR: 0.28, 95% CI: 0.10, 0.79; p = 0.016; FDR q-value = 0.051), respectively, compared to participants who did not use contraceptives. CONCLUSION: These findings suggested that lower serum vitamin D levels were associated with an increased risk of developing BV. However, further studies are needed to confirm the results of the present study.

Comparing the Effect of Acupressure at the Spleen-10 (Xuehai) Acupoint and Vitamin E on Primary Dysmenorrhea.

Objective: Vitamin E and acupressure are 2 complementary and alternative therapies recommended for primary dysmenorrhea (PD). This study assessed the effect of acupressure at Spleen-10 (SP-10), compared to vitamin E on the pain severity of primary dysmenorrhea (PD). Materials and Methods: A quasiexperimental study was conducted on 70 eligible young female students with self-reported primary dysmenorrhea at Gonabad University of Medical Sciences, Gonabad, Iran. Participants were assigned to 2 treatment groups: acupressure or vitamin E. Interventions were implemented at the onset of menstrual pain in each patient, for 2 consecutive periods: acupressure group (n = 35) received 20 minutes of acupressure to SP-10 on both legs; and vitamin E group (n = 35) took 1 capsule of 200 international units of vitamin E. Pain severity was assessed with a visual analogue scale for pain. Data were analyzed with a response-profile analysis in R, version 3.6.1. Significance was P < 0.05. Results: Both treatment groups had a similar pattern of change over time. The mean level of response profiles for pain severity was the same for both groups on all occasions. The effect of time was statistically significant (P < 0.001). A decreasing trend was noted in mean severity of pain over time. Changes at the second and third measured times were significant (P < 0.001). Conclusions: It seems that acupressure at SP10 point is an efficient nonpharmacologic, cost-effective, easy-to-learn way to treat primary dysmenorrhea, applicable in any time or place.

Knowledge Level of Health Care Providers about Complicated Grief during the COVID-19 Pandemic.

Objective: The coronavirus 2019 (COVID -19) pandemic had great psychological impact on COVID-19 patients and their families. Relatives of the deceased COVID-19 patients are at risk for complicated grief. Healthcare providers (HCPs) should be able to identify complicated grief cases. The aim of this study was to assess HCP knowledge regarding complicated grief during the COVID-19 pandemic. Method : This cross-sectional study was conducted using an online researcher-made questionnaire. The questionnaire was designed and validated before being used in this study. The questionnaire included demographic questions as well as knowledge about complicated grief and its symptoms, risk factors and management. The link to the questionnaire website was sent to HCP governmental and private sectors. Data was analyzed using the ordinal regression model by the SPSS 16 software. Results: A total of 887 HCPs (69% female and 31% male) participated in this study. Majority of the participants (594, 70%) had fair overall knowledge about complicated grief while 206 (23.2%) participants had poor knowledge. Poor knowledge level about risk factors for complicated grief was observed in 44.3% of the participants. Fair or poor knowledge about prevention and management of complicate grief was observed in 39.2% of participants. Knowledge about complicated grief had a significant positive relationship with female gender (OR: 1.55; 95% CI: 1.15-2.08) and higher education level (OR: 1.86; 95% CI: 1.37-2.54). Conclusion: Knowledge of HCPs about complicated grief was low. There is need for HCP knowledge improvement regarding complicated grief by appropriate education.

Predictors of the Social Distancing Behaviors during the COVID-19 Pandemic using Protection Motivation Theory in Iran: A cross sectional study.

Background: Social distancing is a key behavior to minimize Coronavirus disease 2019 (COVID-19) infections. Since the change of behavior is the only way to prevent this pandemic, this study aimed to predict the social distancing behaviors during the COVID-19 pandemic using protection motivation theory (PMT). Methods: This cross-sectional study was conducted through a convenience sampling method on 796 individuals over 15 years old from urban and rural areas of different cities in Iran during 2020. The data were collected online using demographic characteristics form, PMT and social distancing behaviors questionnaires. Afterward, the obtained data were analyzed in SPSS software (version 16) through linear correlation coefficient and hierarchical regression tests. Results: The Mean ± SD score of social distancing behaviors was obtained at 4.42 ± 0.31. The results of the hierarchical linear regression model showed that after adjusting the effect of socio-demographic variables, self-efficacy (Beta = 0.238, P < 0.001) was the strongest predictor of social distancing behaviors during the COVID-19 pandemic, followed by intention (Beta = 0.233, P < 0.001) and perceived severity (Beta = 0.083, P = 0.028). PMT constructs and intention was able to predict 40% of social distancing behaviors in total. Conclusions: In the prevalence of infectious diseases, individuals differ in adherence to social distancing behaviors. The PMT was a useful framework for prediction social distancing behaviors during the COVID-19 pandemic. Therefore, this theory can be used as a framework in designing educational programs to increase self-efficacy and encourage individuals to observe social distancing behaviors as a result.

Relationship between Socio-Demographic Factors and Adherence to Social Distancing Recommendations During Covid-19 Pandemic in Gonabad, Iran: A Cross-Sectional Study.

Background: Adherence to social distancing recommendations provided by health authorities plays an important role in the prevention and control of COVID-19 transmission, regarding the lack of effective drug therapies. Therefore, this study aimed to identify the relationship between socio-demographic factors and adherence to social distancing recommendations during COVID-19 pandemic in Gonabad, Iran. Methods: In this cross-sectional study, 587 subjects aged 15 and older were selected using the convenience sampling method from urban and rural areas in Gonabad, Iran, from July to September 2020. Data were collected online using a socio-demographic characteristics form, the adherence to social distancing recommendations scale, and a coronavirus disease awareness survey. Subsequently, the obtained data were analyzed in SPSS software (version 16) using hierarchical linear regression. The statistical significance was considered as P<0.05. Results: 72.23% (95% confidence interval: 68.41%-75.82%) of the participants followed the recommendations of social distancing at the high level. Nevertheless, many differences were observed in the subgroups which resulted from the socio-demographic characteristics of the participants. According to the multiple linear regression model, sex (ß=0.24, P<0.001) was the most important determinant of adherence to social behavioral recommendations, followed by occupation (ß=0.15, P<0.001), age (ß=0.14, P=0.007), awareness (ß=0.13, P=0.001), and history of mental illness (ß=-0.08, P=0.049). Conclusion: For the effective management of pandemics, the general public health policies should also consider the variability in responses and behavioral changes caused by socio-demographic characteristics, rather than focusing only on general measures, such as social distancing.

COVID-19 anxiety and quality of life among Iranian nurses.

BACKGROUND: The Coronavirus Disease 2019 (COVID-19) pandemic has exposed nurses, who are a very important group involved in the care of these patients, to many stresses that may affect their quality of life. This study aimed to determine the relationship between COVID-19 anxiety and the quality of life among Iranian nurses. METHOD: This online cross-sectional study enrolled 1,131 of Iranian nurses working at the time of the COVID-19 outbreak in treatment centers in different parts of Iran from April to May 2020. The convenience sampling strategy was used. Data were collected using a demographic questionnaire, the 36-Item Short-Form Health Survey (SF-36), and Corona Disease Anxiety Scale (CDAS). The stepwise multiple linear regression models were used to examine the relationships among self-reported anxiety concerning COVID-19 and SF-36 quality of life, its components, and subscales. Partial r was used as an estimate of effect size. RESULT: The mean SF-36 score was 65.2 (SD=17.6). The mean score of the mental component summary (MCS) (M=56.8, SD=22.3) was lower than the mean score of the physical component summary (PCS) (M=71.6, SD=17.5). The mean score of COVID-19 anxiety was 17.8 (SD=10.5). Of the participants, 378 (33.4%; 95% CI [30.7%, 36.3%]), and 152 (13.4%; 95% CI [11.5%, 15.6%]) reported moderate and severe anxiety, respectively. According to the results of stepwise multiple linear regression model, after adjusting for possible confounding variables, the SF-36 quality of life was still significantly negatively associated with COVID-19 anxiety, with a large effect size (The partial r = -0.515, p < 0.001). The relationship between the SF-36 components and COVID-19 anxiety were also significant, and moderate to large effect sizes were observed (The partial r for (PCS; COVID-19 anxiety) = -0.404; p < 0.001, and for (MCS; COVID-19 anxiety) = -0.521; p < 0.001). In addition, significant correlation coefficients for every subscale of the SF-36 were found for COVID-19 anxiety and its two components, with small to large effect sizes (The partial correlations= -0.211 to -0.524, all ps< 0.001). CONCLUSIONS: The results showed that higher COVID-19 anxiety in nurses decreases their quality of life. In order to increase nurses' quality of life during the COVID-19 pandemic, it is recommended to design and implement programs to reduce their COVID-19 anxiety.

Relationships between anxiety induced by COVID-19 and perceived social support among Iranian pregnant women.

BACKGROUND: The rapid spread of COVID-19 and the time needed to develop a vaccine or definitive treatment for the disease have caused great anxiety in communities, especially in pregnant women whose high levels of distress may have short and/or long-term maternal and fetal consequence. This study was conducted to investigate the relationship between anxiety induced by COVID-19 and perceived social support in Iranian pregnant women. METHODS: This online cross-sectional study was conducted on 801 pregnant women from all over Iran in 2020. The data were collected using a demographic questionnaire, the multidimensional scale of perceived social support, and the coronavirus disease anxiety scale. A standard multiple linear regression model was used to identify the association between perceived social support and anxiety, controlling for possible confounding variables. Partial r was used as an estimate of effect size. RESULTS: The mean anxiety score was 10.7 ± 8.0, in which its level was moderate in 122 (15.2%; 95% confidence interval (CI): 12.8-17.9%)) of the participants, and severe in 28 (3.4%; 95% CI: 2.3-5.0%) of them. The mean score of perceived social support was 48.2 ± 7.6. 6.9% (95% CI: 5.2-8.8%) and 93.1% (95% CI: 91.2-94.8%) of the participants reported mild and moderate levels of perceived social support, respectively. The results of the multiple linear model showed a significant negative correlation between perceived social support and anxiety levels in a way that for every 10 units increase in the perceived social support score, the anxiety level of pregnant women was decreased by 0.8 units (B= -0.08, t= -2.08, p = 0.037), which was a small effect size (partial r = -0.07). CONCLUSION: There was a small significant relationship between the perceived social support and COVID-19 anxiety. Further studies are required to identify associated factors of anxiety level during COVID-19 in pregnant women.

A review on advances in the applications of spider silk in biomedical issues.

Spider silk, as one of the hardest natural and biocompatible substances with extraordinary strength and flexibility, have become an ideal option in various areas of science and have made their path onto the biomedical industry. Despite its growing popularity, the difficulties in the extraction of silks from spiders and farming them have made it unaffordable and almost impossible for industrial scale. Biotechnology helped production of spider silks recombinantly in different hosts and obtaining diverse morphologies out of them based on different processing and assembly procedures. Herein, the characteristics of these morphologies and their advantages and disadvantages are summarized. A detailed view about applications of recombinant silks in skin regeneration and cartilage, tendon, bone, teeth, cardiovascular, and neural tissues engineering are brought out, where there is a need for strong scaffolds to support cell growth. Likewise, spider silk proteins have applications as conduit constructs, medical sutures, and 3D printer bioinks. Other characteristics of spider silks, such as low immunogenicity, hydrophobicity, homogeneity, and adjustability, have attracted much attention in drug and gene delivery. Finally, the challenges and obstacles ahead for industrializing the production of spider silk proteins in sufficient quantities in biomedicine, along with solutions to overcome these barriers, are discussed.

The prevalence of preventive behaviors and associated factors during the early phase of the COVID-19 pandemic among Iranian People: Application of a Health Belief Model.

INTRODUCTION: Coronavirus disease 2019 (COVID-19) has developed into a severe public health concern. The present study was aimed to evaluate the related preventive behaviors to COVID-19, and associated factors among Iranians based on the constructs of the Health Belief Model (HBM). METHODS: This cross-sectional study was conducted between March and April 2020 among Iranians. Data of 1,020 people were collected by a self-administered questionnaire based on the constructs of HBM and also a demographics questionnaire. Simple and multivariable linear regression models were used to determine the predictors of preventive behaviors concerning COVID-19. A P-value of less than 0.05 was considered significant. RESULTS: The mean score of preventive behaviors concerning COVID-19 was 4.27 (standard deviation = 0.60). 40.6% (95% CI: 38.6-43.7%), and 56.5% (95% CI: 53.4-59.5%) of the participants exhibited a high, and moderate level of preventive behaviors, respectively. 75.1% of the participants would always/often wear face masks out home or in crowded places. The most common preventive behaviors were using of personal belongings in the workplace or at home (96.1%) and staying at home except for very necessary or for work (92.9%). Results showed that all six HBM constructs, i.e., perceived susceptibility (B = 0.07, P < 0.001), perceived severity (B = 0.08, P < 0.001), perceived benefits (B = 0.25, P < 0.001), perceived barriers (B = -0.12, P = 0.001), cues to action (B = 0.07, P < 0.001), and self-efficacy (B = 0.26, P < 0.001) were significant predictors of related preventive behaviors to COVID-19. Female gender was another predictor of preventive behaviors (B = 0.18, P < 0.001). CONCLUSIONS: This study demonstrated the effectiveness of HBM constructs in predicting COVID-19 preventive behaviors. Therefore, the model as a framework for designing training programs for improving health behaviors among Iranians during the COVID-19 pandemic seems to be helpful.

Effect of shared decision making on mode of delivery and decisional conflict and regret in pregnant women with previous cesarean section: a randomized clinical trial.

BACKGROUND: The promotion of vaginal birth after cesarean section (VBAC) is the best method for the reduction of repeated cesarean sections. Nonetheless, the decisional conflict which often results from inadequate patient involvement in decision making, may lead to delayed decision making and regret about the choices that were made. The present study aimed to determine the effect of shared decision making on the mode of delivery and decisional conflict and regret in pregnant women with previous cesarean section. METHODS: This randomized clinical trial was conducted on 78 pregnant women with a previous cesarean section referring to community health centers in Torbat-e Jam, Iran, in 2019. They were randomly assigned to two groups of intervention and control. During weeks 24-30 of pregnancy, the Decisional Conflict Scale (DCS) was completed by pregnant mothers. Apart from the routine care, the experimental group received a counseling session which was held based on the three-talk model of shared decision making. This session was moderated by a midwife; moreover, a complementary counseling session was administered by a gynecologist. During weeks 35-37 of pregnancy, DCS was completed, and the Decision Regret Scale (DRS) was filled out for both groups at the 8th weeks postpartum and they were asked about the mode of delivery. Data were analyzed in SPSS software (version 19) using the Mann-Whitney, Chi-squared and Fisher's exact tests. p-value less than 0.05 was considered statistically significant. RESULTS: After the intervention, the decisional conflict score was significantly lower in the shared decision making (SDM) group, compared to that in the control group (14.90 ± 9.65 vs. 25.41 ± 13.38; P < 0.001). Moreover, in the SDM group, the rate of vaginal birth was significantly higher than that in the control group (P < 0.001). Two month after the delivery, the mean score of decision regret was lower in the SDM group, in comparison to that in the control group (15.67 ± 23.37 vs. 27. 30± 26.75; P = 0.007). CONCLUSIONS: Based on the results of the study, shared counseling can be effective in the reduction of decisional conflict and regret, as well as rate enhancement of VBAC. Therefore, it can be concluded that this counseling method can be used in prenatal care to reduce the rate of repeated cesarean section. TRIAL REGISTRATION: IRCT20190506043499N1; Name of the registry: Iranian Registry of Clinical Trials; Registered 10. August 2019. URL of registry: https://en.irct.ir/trial/39538. Date of enrolment of the first participant to the trial: August 2019.

Anxiety Severity Levels and Coping Strategies during the COVID-19 Pandemic among People Aged 15 Years and Above in Gonabad, Iran.

BACKGROUND: The coronavirus disease 2019 (COVID-19) outbreak is a global health problem. It is necessary to provide evidence on its unprecedented psychological effects to develop effective psychological interventions. The current study aims to determine the anxiety severity level, coping strategies, and influencing factors in response to the COVID-19 pandemic among people aged 15 years and above in Gonabad, Iran. METHODS: We conducted a cross-sectional survey via online questionnaires between February and March 2020. We evaluated the anxiety severity levels and coping strategies using the Corona Disease Anxiety Scale (CDAS) and Coping Inventory for Stressful Situations-Short Form (CISS-SF), respectively. Multinomial and ordinal logistic regression models were used to identify the predictors of coping strategies and anxiety. RESULTS: Totally, 500 people completed the questionnaires (response rate: 73%). Of them, 53.4% (95% confidence interval [CI]: 48.9%- 57.8%) suffered moderate to severe levels of anxiety. More than half of the respondents (52.0%; 95% CI: 47.5%-56.4%) utilized emotional-based or avoidant coping strategies. People with no academic education (odds ratio [OR]: 2.16; 95% CI: 1.41- 3.31) and without physical exercise (OR: 2.04; 95% CI: 1.22-3.33) preferred emotional-based coping instead of problem-based coping strategy. Female gender (OR: 1.60, 95%, CI: 1.13-2.28), underlying medical conditions (OR: 2.52, 95% CI: 1.65-3.87), and emotional-based coping (OR: 4.06, 95% CI: 2.76-5.99) were associated with higher severity levels of anxiety. CONCLUSION: The severity of anxiety during the COVID-19 pandemic was significant among participants. Further attention is needed to enhance the mental health of the vulnerable population during the COVID-19 pandemic. Our findings also identified some factors related to the severity level of anxiety related to COVID-19 that could help formulate better psychological interventions.

Early oleate deficiency leads to severe defects in fetal rat liver development.

OBJECTIVES: Oleate can be produced through de novo synthesis, which contributes to biological processes and signaling pathways. However, the role of this non-essential fatty acid in hepatic development remains unclear. The current study aimed to evaluate the influence of early oleate deficiency induced by the inhibitor of de novo oleate synthesis MF-438 on fetal rat liver development. MATERIALS AND METHODS: Female Wistar rats with an average weight of 200±20 g were subjected to this study. After mating, pregnant rats were divided into three groups and gavaged with the vehicle, MF 438 or MF-438 plus oleate from day 3 of pregnancy for five days. Obtained fetuses were sacrificed and the liver tissues were retrieved. Hepatic morphological index, biochemical markers, and gene expression of hepatic development markers were analyzed using Hematoxylin-Eosine, spectrometry, and real-time PCR techniques, respectively. RESULTS: Relatively, deficient morphological indices and hepatic maturation markers were observed in fetus livers of the inhibitor-treated group. In comparison to the other two groups, total hepatic protein and glycogen content were increased with treatment of MF-438 plus oleate. Hepatocyte nuclear factor 1α, alpha fetoprotein, albumin, and cytochrome P450 gene expression were also significantly increased in the group treated with both MF-438 and oleate. CONCLUSION: Our data indicate that oleate availability during early embryo development is linked with fetal rat liver development.

Identification of associated risk factors for the severity of generalized anxiety disorder among Iranian infertile people: An ordinal regression analysis with a flexible link function.

BACKGROUND: Generalized anxiety disorder (GAD) is a common disorder in infertile people. The aim of this study was the identification of associated risk factors for the severity of GAD in infertile people using an ordinal model with a flexible link function. MATERIALS AND METHODS: This cross-sectional study was conducted on 1146 individuals with a couple's infertility problem selected from an infertility center in Tehran, Iran. Data collected using self-administered questionnaires include demographic/clinical information and GAD-7. We used a Bayesian-ordered symmetric power logit (splogit) model to identify the risk factors for the severity of GAD. Furthermore, we implemented standard ordinal models to compare with the ordered splogit model. RESULTS: Female gender (B coefficient 0.48, 95% credible interval [CrI]: 0.34-0.62), longer duration of infertility (B coefficient 0.03, 95% CrI: 0.01-0.04), previous treatment failure (B coefficient 0.17, 95% CrI: 0.03-0.30), and self-cause of infertility (B coefficient 0.12, 95% CrI: 0.01-0.23) were associated factors with the severity of GAD. The splogit model had a better fit and performance to determine the associated risk factor for the severity of GAD as compared to standard models. It provided more precise estimates of risk factors and one more significant risk factor. CONCLUSION: Infertile people with female gender, longer duration of infertility, failure in previous treatments, and self-cause infertility are more likely to experience higher severity levels of GAD and require additional psychological, and support interventions. Furthermore, it can be argued that the ordinal splogit model is more powerful to identify the associated risk factors for the severity of GAD.

Chronic musculoskeletal pain, phantom sensation, phantom and stump pain in veterans with unilateral below-knee amputation.

BACKGROUND AND AIMS: Many individuals with lower limb loss report concerns about other musculoskeletal symptoms resulting from amputation. The objective of this study was to assess chronic musculoskeletal pain in Iranian veterans with unilateral below-knee amputation. METHODS: The participants agreed to take part in a health needs assessment and were interviewed face-to-face by trained interviewers. The assessment consisted of demographic information, wearing a prosthesis, pain locations in extremities, stump complications, severity of pains related to amputation and low back pain. RESULTS: Of 247 unilateral below knee amputees, 97.9% wore a prosthetic limb and times walking or standing with the prosthesis were 12.47 ± 3.84 and 4.22 ± 3.53 h a day, respectively. Low soft tissue coverage of the stump (15.4%) and symptomatic osteoarthritis in the contralateral lower extremity (40.1%) were the most common complications. The prevalence of stump pain, phantom sensations, phantom pain, low back pain, and knee pain was 84.2%, 77.3%, 73.7%, 78.1%, and 54.7% respectively. The odds ratio of stump pain in amputees with phantom pain was 2.22 times higher than those who did not experience phantom pain [OR = 2.22 (CI: 1.19-4.17); p = 0.012] and the odds ratio of low back pain was higher in amputees with stump pain [OR = 3.06 (CI: 1.50-6.21); p = 0.002]. CONCLUSIONS: This research enhances our understanding of comorbid musculoskeletal problems in below-knee amputees which can help health providers to identify rehabilitation needs and emphasizes the importance of regular assessments. IMPLICATIONS: These findings underline the importance of paying closer attention to different dimensions and aspects of musculoskeletal complications in veterans with unilateral below-knee amputation.

Correlation between expression of CatSper1,2 and sperm parameters in the gamma irradiated adult mouse testis.

Propose: CatSper protein channels are responsible for the entry of Ca2+ into sperm cells. These proteins play an important role in motility and male fertility. So it is important to find out whether or not environmental factors, such as gamma radiation, have an effect on the expression of Catsper genes. In this study, we investigated the effects of gamma radiation on the expression of CatSper1 and CatSper2 genes. Materials and methods: Twenty-one male NMRI mice were divided into three groups: a control group without gamma radiation, and two experimental groups; Group 1 treated with 1 Gy of gamma radiation, and Group 2 treated with a higher dose of 2 Gy gamma radiation. Testes were removed from all groups of animals 35 days following irradiation and the testicular tissue, processed and embedded in paraffin blocks for sectioning and histological examination. Sperm samples were also taken from the epididymis for microscopic. Sperm parameters such as sperm count, morphology, motility, and viability rates were analyzed. Expression of CatSper genes was evaluated using Real-time PCR. Data were analyzed using the SPSS software and ANOVA test. Results: Our results showed that after treatment with gamma radiation, testes morphology was changed. Epididymal sperm count, motility, and morphology rates were significantly affected in both experimental groups compared to the control group. The relative expressions of CatSper 1 and 2 genes were significantly reduced in the irradiated mice (1 Gy and 2 Gy) than non-irradiated ones. Conclusions: Gamma radiations not only change testes histology and sperm parameters, but also decrease the expression of CatSper 1 and 2 genes in male mice.